Purification and properties of glutathione peroxidase from Mucor hiemalis.
نویسندگان
چکیده
منابع مشابه
Purification and properties of glutathione peroxidase from human placenta.
Glutathione peroxidase (glutathione--H2O2 oxidoreductase; EC 1.11.1.9) was purified to homogeneity from human placenta by using (NH4)2SO4 precipitation, ion-exchange chromatography, Sephadex gel filtration and preparative polyacrylamide-disc-gel electrophoresis. Glutathione peroxidase from human placenta is a tetramer, having 4g-atoms of selenium/mol of protein. The molecular weight of the enzy...
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Previous studies (1,2) have shown the presence of a peroxidase in erythrocytes which catalyzes the breakdown of hydrogen peroxide with reduced glutathione serving as a hydrogen donor. The significance of this enzyme in the protection of hemoglobin from oxidative breakdown has been shown previously (2). The erythrocyte reduced glutathione peroxidase has now been purified by column chromatography...
متن کاملPurification and properties of human erythrocyte glutathione peroxidase.
Glutathione peroxidase has been purified to homogeneity from human erythrocytes. The purification steps involved ammonium sulfate precipitation of hemolysate, CM-cellulose (CM-52), DEAE-cellulose (DE52), Sephadex G-200, and DEAE-Sephadex column chromatography. In the last step, i.e. DEAE-Sephadex A-25 column chromatography, the enzyme was eluted in a major peak and tailing fraction. The major p...
متن کاملPurification and characterization of a glutathione S-transferase from Mucor mucedo.
An intracellular glutathione transferase was purified to homogenity from the fungus, Mucor mucedo, using DEAE-cellulose ion-exchange and glutathione affinity chromatography. Gel filtration chromatography and SDS-PAGE revealed that the purified GST is a homodimer with approximate native and subunit molecular mass of 53 kDa and 23.4 kDa, respectively. The enzyme has a pI value of 4.8, a pH optimu...
متن کاملSubcutaneous zygomycosis caused by Mucor hiemalis in an immunocompetent patient.
Zygomycosis is an opportunistic fungal infection with a high mortality rate. It is known to cause invasive disease in immunocompromised hosts but it may produce only cutaneous/ subcutaneous infections in immunocompetent hosts. Treatment is difficult due to its fulminant course and lack of effective anti-fungal drugs. Here, we report a rare case of subcutaneous zygomycosis caused by Mucor hiemal...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1983
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.47.1107